On Top 10 Medical Innovations for 2021 (selected by Cleveland Clinic, Cleveland, USA), Gene therapy was successfully selected. Cell and gene therapy has changed the way humans treat genetic diseases and difficult conditions, and some landmark studies have pushed cell and gene therapy to the pharmaceutical industry. The rapid development of this field has also brought significant challenges, such as the conquest of viral vectors, how to increase the efficiency of gene transfer or editing to the level necessary to effectively treat diseases, the long-term monitoring of patients after gene therapy, and the development of new technologies based on the limitations of existing biotechnology to solve some of the problems currently faced.
Digital PCR is a new generation technique for the absolute quantification of nucleic acid molecules. Compared with qPCR, digital PCR can directly count the number of differential regions containing DNA molecules and perform absolute quantification of the starting sample by Poisson distribution algorithm. It has the advantages of high sensitivity, high precision, and high tolerance to inhibitors. In the field of cell and gene therapy, digital PCR can be used for copy number quantification of viral genomes, absolute quantification of standards, microbial fast detection, and host DNA or RNA residue detection.
Beijing Cycloudbio Mr. Bing Li, Digital PCR Product Manager, presented "Application and Practice of Digital PCR Methods in Cell and Gene Therapy", focusing on the parallelism and conversion of digital PCR methodology in the field of cell and gene therapy, to better understand the digital PCR technology and its application in the field of gene and cell therapy for clinical and research workers. The workshop will focus on the parallelism and conversion of digital PCR methodology in the field of cell and gene therapy.
Conference Info
Time: March 19-20, 2021
Venue: Shanghai, China
The conference covers areas: cell therapy, gene therapy, innovative therapies, lysing viruses, CDMO, CRO, regulations, IND applications, GLP, clinical trial design, GMP, immunomodulation, viral vectors, high throughput screening, transfection, combination therapy, IVD, digital PCR, CRISPR/CAS9 gene editing, humanized animal models, mRNA tumor vaccines nanobodies, BsAbs, tumor microenvironment, stem cell therapy, recombinant proteins, etc.
Booth Location:A12
Booth product display
naica®Next Generation Digital PCR
naica® crystal Digital PCR system uses sapphire chip as the only consumable to form a 2D array of 25,000-30,000 droplets for PCR amplification experiments in a single-layer tile method. After the completion of the reaction, three channel imaging is performed to obtain the accurate absolute number of nucleic acids. Results can be obtained quickly within 2.5 hours.
▲ naica®crystal Digital PCR system
Azure Cielo™ Real-Time PCR System
Azure Cielo real-time fluorescence quantitative PCR system comes from Azure Biosystems of the United States, whichcombines high-quality PCR temperature module and excellent fluorescence detection system to provide high-precision, highly sensitive and reliable results for your scientific research.
▲ Azure Cielo™ Real-Time PCR System
Echo Hybird microscope
Echo Revolve microscopy basics has the functions of both upright and inverted microscope, which is convenient andcompact, multi-functional, can easily switched between upright and inverted modes; replaces the traditional eyepiece design with advanced for control, observation, transmission, image acquisition and management, while the IOS based Echo app makes the software operation more humanized.
▲ Echo Revolve Hybird microscope
▲ Echo Rebel Hybird microscope