[Invitation] Gene π Practical Classroom-Digital PCR Molecular Diagnosis Method Establishment and Verification

With the accumulation of molecular genetic knowledge, the quantification of DNA and RNA molecules has become more and more important. Various types of genomics technologies emerge in an endless stream, and new molecular markers and molecular diagnostic technologies continue to emerge. Molecular diagnostics are currently not only used in clinical practice, but also play an increasingly important role in disease prevention, screening and other fields. The Gene-π Digital PCR Learning Center of the 4th Clinical Molecular Diagnosis forum-the establishment and verification of digital PCR molecular diagnostic methods will be held in Shanghai on December 3, 2020. This training camp is sponsored by Zhongshan Hospital Affiliated to Fudan University, China Medical Biotechnology Association Genetic Testing Technology Branch, Translational Medicine Network, and co-organized by Beijing Cycloud Biotechnology Co., Ltd. and Apexbio Biotechnology (Suzhou) Co., Ltd.

Molecular diagnosis is a branch of medicine that applies molecular biotechnology to disease diagnosis. It uses molecular biology technology to study the existence, structure or expression regulation changes of human endogenous or exogenous biomolecules. It provides information and decision-making basis for disease prevention, prediction, diagnosis, treatment, prognosis and outcome.Digital PCR is currently one of the most important nucleic acid molecular diagnostic techniques.

Digital PCR (digital PCR, dPCR) technology is a brand-new technical means for accurately quantifying nucleic acid molecules. It adopts an absolute quantification method and does not rely on standard curves and reference samples. It directly detects the copy number of the target sequence with high sensitivity and specificity. Digital PCR has shown good application prospects in many clinical fields, such as tumor liquid biopsy, organ transplant damage assessment, non-invasive prenatal screening, molecular diagnosis of pathogenic microorganisms, and quality control and result verification of second-generation sequencing libraries, etc. In advanced therapy, such as cell therapy, gene therapy, immunotherapy, the quality control system is very important, and the absolute quantification of target nucleic acids helps intuitive and effective index quantification.

Digital PCR improves the resolution of low-frequency events and has advantages of greater inhibitor tolerance. In all aspects of translational medicine, life sciences, and biological sciences, there are innovative research and applications. And it has multiple fluorescence channels and visual quality control features. It can perform specific detection of a variety of genes, and ensure that more reliable data is obtained quickly, enabling more information to be obtained in the case of limited biological samples, and significantly improving detection capabilities and work efficiency.

In order to better understand the digital PCR technology for the majority of scholars, the training camp will invite senior technical experts to talk about detailed explanation of third-generation digital PCR technology, experimental operation, result analysis, molecular diagnostic method establishment and verification, quantification of advanced treatment quality control system, liquid biopsy, quantitative application case analysis of standard products.

Organizer

Zhongshan Hospital Affiliated to Fudan University

Genetic Testing Technology Branch of China Medical Biotechnology Association

Translational Medicine Network

Co-organiser

Beijing Cycloud Biotechnology Co., Ltd.

Apexbio Biotechnology (Suzhou) Co., Ltd.

Time and Venue

Time：2020年12月03日

Venue：Zongluo Hall, Good Hope Hotel, Shanghai

Training schedule