Use KeyPro Decontamination System to prevent degradation of RNA and ensure the accuracy of PCR results

Nucleic acid testing plays an important role as the gold standard for confirmatory diagnosis new coronavirus. As everyone knows, the nucleic acid detection process of this new coronavirus is extracting viral RNA, and then detecting the sample by RT-qPCR or one-step RT-digital PCR. However, recent discussions on the accuracy of nucleic acid detection are also very hot point. PCR experiment contamination is one of the reasons for the deviation of the experiment. The main causes of PCR experiment contamination are the following：Cross-contamination between samples ，contamination of PCR reagents (contamination of containers, consumables, pipettes, water, etc.), contamination of PCR amplification products, aerosol contamination, cloning plasmid contamination, etc.

In the process of viral nucleic acid detection, in addition to the sensitivity of the detection method is important, ensuring the integrity of the extracted RNA samples is also very important. Today we will talk about how to prevent RNA degradation and obtain high-quality RNA for experiments. The main substance that causes RNA degradation is RNase. RNase is very stable and ubiquitous. All conventional RNase methods and protease inhibitors cannot completely inactivate all RNases.

RNase

In addition to the above methods, researchers commonly use DEPC to treat consumables and reagents , but DEPC processing is long and toxic. Here are three reasons to stay away from DEPC:

1、DEPC is harmful to the human body. DEPC is a highly active alkylating agent that can covalently modify proteins .At the same time, it can make unpaired adenine methylolate and change its activity. DEPC is strongly carcinogenic，Be very careful when using.

2、Some reagents cannot be processed: for example: such as TRIS, PBS, etc., chemical reactions will occur; reagents that cannot be autoclaved, such as DTT, dNTP, Mgcl2, because DEPC must be removed by autoclaving; It cannot be used for reagents with pH lower than 4 and below. DEPC has no inactivation effect on RNase under these conditions.

3、DEPC is not suitable for some experiments. The OD is related to pH，DEPC is decomposed in solution into ethanol and carbon dioxide. The carbon dioxide reacts with water to form carbonic acid, which causes the pH to decrease and reduces the OD value.

Experimenters urgently need a very fast and convenient method to remove RNase to ensure the accuracy of the results. Using KeyPro ™ KP100 Decontamination System the pollution problems can be eliminated in 5min through the synergistic effect of 275nm and 365nm dual UV.KeyPro™ KP100 eliminates contamination without residue or rinsing, saving up to 90% of the time and cost of chemical decontamination. Simply load compatible reagents and solvents and run your decontamination cycle just prior to adding sample.

KeyPro™KP100 Decontamination System uses SLM ™ patented technology to integrate LED array, optical components and thermal cooling technology, which can maximize the disinfection and decontamination performance, saving your research time.

Instrument advantage:

1. 5min completely kills viruses, bacteria etc

2. Touch screen operation, easy to use.

3. Fast and high throughput.

4. Stable and consistent results.

5. Reduce the cost of laboratory consumables.

6. Integrated workflow