On October 16th, and October 30th, 2015, Azure Biosystems China technical demonstration and service center presented the “Membrane Legend —- Western Blot’s Mystery” at Heilongjiang Jiamusi University, and Tianjin Agricultural University, respectively, receiving enthusiastic feedbacks from fellow teachers, and students.
Such seminars employed technological-interaction method, focusing on discussing operational precautions and trouble-shooting of western blot, as well as the selection of multiple quantitative western blot. Chemiluminescence method, together with Super-sensitive Chemiluminescence Substrate, is able to achieve fg level protein, which has highly sensitive detection. However, its illumination is controlled by the response of enzyme catalysis. Therefore, during multi-protein quantified study, we suggest choosing multicolor fluorescence (RGB) and near-infrared light (NIR), mark secondary antibody as positive, no need to be stripped, stable signal, quantified accuracy. Moreover, when near-infrared light is marked as secondary antibody during testing, background noise is extremely low, significantly increases the sensitivity of the test.
Chemiluminescence and florescence are both the favorites in the world of membrane protein. Above all, Azure Biosystems China technical and service center is willing to continue the biological legend with you.
If you want to know more about “Western Blot’s Mystery,“ and become an excellent “protein membrane magician,” please follow our wechat: “Azure蛋白印像” for contact information.